Construction of an expression vector carrying gene coding mannitol-1-phosphate dehydrogenase (mtlD) from Escherichia coli JM109 strain to transfer into maize
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D-Mannitol 1-phosphate dehydrogenase from Escherichia coli.
D-Mannitol is the most widely distributed of the naturally occurring hexahydric alcohols, and the compound has been known since the early part of the nineteenth century. It is found as a reserve material in some algae and many higher plan&. In some lower fungi it is the product of the fermentation of sugars. Finally, mannitol is utilized for growth and fermented by a large number of species of ...
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Galactitol-1-phosphate 5-dehydrogenase (GPDH) is a polyol dehydrogenase that belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily. It catalyses the Zn(2+)- and NAD(+)-dependent stereoselective dehydrogenation of L-galactitol 1-phosphate to D-tagatose 6-phosphate. Here, three crystal structures of GPDH from Escherichia coli are reported: that of the open state of GPDH with Zn(2+...
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The presence of a mannitol cycle in fungi has been subject to discussion for many years. Recent studies have found no evidence for the presence of this cycle and its putative role in regenerating NADPH. However, all enzymes of the cycle could be measured in cultures of Aspergillus niger. In this study we have analyzed the localization of two enzymes from the pathway, mannitol dehydrogenase and ...
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Hepatitis B surface antigen is the first genetically engineered vaccine licensed for human use. Various strategies have been proposed to obtain a vaccine that would bypass the need for injection. In this study, a non-toxic portion of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface antigen. T...
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New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla(NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla(NDM-1), bla(CTX-...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2016
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v39n1.7073